Escherichia Coli: Characteristics of Carbapenem Resistance and Virulence Factors
AUTOR(ES)
Candan, Esra Deniz, Aksöz, Nilüfer
FONTE
Braz. arch. biol. technol.
DATA DE PUBLICAÇÃO
22/06/2017
RESUMO
ABSTRACT In this study, fifty Escherichia coli strains were analyzed by multiplex polymerase chain reaction for the genes expressed carbapenemase and virulence factors in order to determine the presence of carbapenemase and nine virulence factors and investigate the association between these two characteristics. When carbapenemase susceptibility was taken into consideration, OXA-48 type carbapenemase was determined for 22% of the total strains. Also, the frequency of virulence gene regions in E.coli infections and virulence gene profiles of these isolates were examined and the frequency of pap, afa, sfa, fimA, iroN, aer, iutA, hly and cnf-1 genes were 24, 38, 20, 84, 28, 90, 92, 10 and 34% respectively. A significant correlation was found between the presence of fimA and afa gene regions and carbapenem susceptibility (P< 0.05). Based on the combination of carbapenemase and virulence factor genes, 24 different gene profiles were determined for all strains. The results of the study appear to indicate that fimA and afa genes correlate with carbapenem susceptibility, the relations of fimA with urinary tract infections and pap with complicated urinary tract infections. It also indicates that sfa and afa genes correlate with other infections except urinary tract infections.
Documentos Relacionados
- Virulence factors and biochemical characteristics of serotypes of Escherichia coli serogroup O29.
- Capsular K1 polysaccharide of Escherichia coli: relationship to virulence in newborn rats and resistance to phagocytosis.
- Role of Virulence Factors in Resistance of Avian Pathogenic Escherichia coli to Serum and in Pathogenicity
- Plasmid-mediated formaldehyde resistance in Escherichia coli: characterization of resistance gene.
- Virulence factors and antimicrobial resistance of escherichia coli isolated from urinary tract of swine in southern of Brazil