Erythrovirus B19 infection in acquired immunodeficiency syndrome: screening by histopathology, immunohistochemistry, and in situ hybridization
AUTOR(ES)
Setúbal, Sérgio, Oliveira, Solange Artimos de, Pires, Andréia Rodrigues Cordovil, Fonseca, Eliene Carvalho da, Camacho, Luiz Antônio Bastos, Seródio, Ana Cristina Freire, Nascimento, Jussara Pereira do
FONTE
Memórias do Instituto Oswaldo Cruz
DATA DE PUBLICAÇÃO
2006-06
RESUMO
Erythrovirus B19 infects erythrocytic progenitors, transiently interrupting erythropoiesis. In AIDS patients it causes chronic anemia amenable to treatment. We looked for evidences of B19 infection in stored bone marrow material from patients with acquired immunodeficiency syndrome. Histological sections were made from stored paraffin blocks from 33 autopsies (39 blocks) and 35 biopsies (45 blocks, 30 patients) performed from 1988 to 2002. They were examined after hematoxylin-eosin (HE) staining, immunohistochemical (IHC), and in situ hybridization. HE revealed intra-nuclear inclusion bodies ("lantern cells") suggesting B19 infection in 19 sections corresponding to 19 of 63 patients examined with this test. Seven of 78 sections subjected to immunohistochemistry were positive, corresponding to 7 of 58 patients examined with this test. Fourteen sections corresponding to 13 of the 20 HE and/or IHC positive patients were subjected to in situ hybridization, with six positives results. Among the 13 patients subjected to the three techniques, only one gave unequivocal positive results in all and was considered a true positive. The frequency of B19 infection (1/63 patients) in the material examined can be deemed low.
Documentos Relacionados
- Transient red cell aplasia in two brothers with sickle cell anemia and erythrovirus B19 infection
- Erythrovirus B19 induced persistent bicytopenia in a healthy child
- Detection of parvovirus B19 DNA in bone marrow cells by chemiluminescence in situ hybridization.
- Detection of parvovirus B19 in macerated fetal tissue using in situ hybridization.
- Persistent parvovirus b19 infection resulting in carpal tunnel syndrome