Epidermal growth factor inhibits the synthesis of the nuclear protein cyclin in A431 human carcinoma cells.
AUTOR(ES)
Bravo, R
RESUMO
The effect of epidermal growth factor (EGF) on the synthesis of the nuclear protein cyclin and its relationship with cell proliferation has been studied in human carcinoma A431 cells. Quantitative two-dimensional gel electrophoretic analysis of [35S]methionine-labeled polypeptides from EGF-treated and untreated A431 cells showed that the synthesis of cyclin (0.07% of total labeled protein) could be decreased to 20% (less than 0.01% of total labeled protein) by EGF at a concentration of 100 ng/ml. A good correlation between the effect of different concentrations of EGF on cyclin synthesis and A431 cell proliferation was found. A431-derived cells resistant to the growth-inhibitory effect of EGF showed no significant changes in cyclin synthesis after EGF treatment. Taken together, these results support the idea that cyclin may be an important protein that is involved in the control of cell proliferation.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=391588Documentos Relacionados
- Aspects of the metabolism of the epidermal growth factor receptor in A431 human epidermoid carcinoma cells.
- Biosynthesis of the epidermal growth factor receptor in A431 cells.
- Down-regulation of epidermal growth factor receptor correlates with plasminogen activator activity in human A431 epidermoid carcinoma cells.
- Structure and localization of genes encoding aberrant and normal epidermal growth factor receptor RNAs from A431 human carcinoma cells.
- Kinetics and regulation of the tyrosine phosphorylation of epidermal growth factor receptor in intact A431 cells.