Enhanced Cellulase Production by a Mutant of Sclerotium rolfsii†
AUTOR(ES)
Sadana, J. C.
RESUMO
A mutant of Sclerotium rolfsii CPC 142 that secretes about two times more filter paper-degrading activity in NM-2 growth medium in submerged cultures than the parent strain was obtained by ultraviolet mutagenesis of crushed sclerotia. The production of endo-β-glucanase in the mutant was affected to a lesser extent. With the parent strain, the addition of 3% rice bran to NM-2 medium was essential for optimal formation of cellulase, including filter paper-degrading activity. However, with the mutant the addition of rice bran to NM-2 medium increased the formation of endo-β-glucanase but not filter paper-degrading or cellobiase activity. An altered control mechanism for the production of filter paper-degrading enzymes is suggested. The genome(s) controlling the cellulase complex of enzymes in the UV-8 mutant is not under coordinate control.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=243567Documentos Relacionados
- Enhanced Cellulase Production by a Mutant of Trichoderma viride
- High Cellobiase and Xylanase Production by Sclerotium rolfsii UV-8 Mutant in Submerged Culture †
- Sensibilidade in vitro de Sclerotium rolfsii a fungicidas
- Reação de genótipos de feijão-fava a Sclerotium rolfsii
- Preparation of mutants of Trichoderma reesei with enhanced cellulase production.
