Energy-linked Sulfate Uptake by Corn Mitochondria via the Phosphate Transporter 1

AUTOR(ES)

Abou-Khalil, Samir

RESUMO

Corn shoot mitochondria possess an energy-linked transport system for sulfate uptake as demonstrated by osmotic swelling and [35S]SO42− accumulation. Maximum uptake is secured in the presence of Mg2+ and oligomycin with sucrose for osmotic support. Neither phosphate nor dicarboxylate anions are required. When added simultaneously, millimolar concentrations of phosphate block [35S]SO42− uptake after the initial minute. Mersalyl, N-ethylmaleimide, and 2,4-dinitrophenol are strong inhibitors of sulfate uptake; n-butylmalonate is a weak inhibitor. These inhibitors act in the same fashion on phosphate uptake. It is concluded that sulfate uptake in the absence of phosphate is by the phosphate transporter.

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