Effects of L-aginine and deflazacort in muscle regeneration after experimental envenoming by Bothrops jararacussu / Efeito da L-arginina e deflazacort na regeneração muscular apos o envenenamento experimental por Bothrops jararacussu

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

The study evaluates the effect of deflazacort (DFZ), an anti-inflammatory oxalazine derivative of prednisone, on muscle regeneration following myonecrosis experimentally induced by B. jararacussu venom. Mice (n=15) right tibialis anterioris muscle was injected with 80 µg of venom. Two groups of animals (n=10) was treated during 5 and 20 days with a daily intraperitoneal injection of deflazacort, an anti-inflammatory oxalazine derivative of prednisone (1,2 mg/kg). The third group was injected with crude venom and did not receive any pharmacological treatment. The animals were killed 5 and 60 days after envenoming and muscle regeneration was evaluated by counting the number of muscle fibers and measuring the fibrosis area. We found that in deflazacort-treated group the area of fibrosis was higher (p<0.05) than in injected muscles without treatment (1.077.051 ± 466.658,2; versus 777.107,3 ± 356.804,8 pixels square), and the number of muscle fiber was significant different too (755 ± 84; versus 1.221 ± 102). Although it does not reach the level of muscle fiber population of uninjured tibialis anterioris muscle (3.257 ± 478). We conclude that deflazacort treatment is detrimental to muscle fiber regeneration aggravating the loss of muscle mass after B. jararacussu envenoming.

ASSUNTO(S)

musculos - regeneração muscle regeneration bothrops jararacussu l-arginina deflazacort l-arginine

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