Effect of seminal plasma and egg yolk concentration on freezability of goat semen
AUTOR(ES)
Ferreira, Valéria da Silva, Mello, Marco Roberto Bourg de, Fonseca, Carlos Elysio Moreira da, Dias, Állan César Ferreira, Cardoso, Jéssica Machado, Silva, Rebecca Barbosa, Martins Júnior, Wagner Pereira
FONTE
R. Bras. Zootec.
DATA DE PUBLICAÇÃO
2014-10
RESUMO
The objective of this study was to evaluate the effects of egg yolk and seminal plasma on the viability of cryopreserved goat semen. To this end, four fertile Saanen bucks, aged between 10 months and 1 year, and weighing 18 to 25 kg, were used. Semen was collected from each buck by the artificial vagina method at the end of breeding season (June-July). The extender used was the yolk citrate, which was split into two equal aliquots: 5% egg yolk (2.5 mL egg yolk: 47.5 mL citrate solution) were added to one of the samples and 10% egg yolk (5.0 mL egg yolk: 45.0 mL citrate solution) were added to another. The sperm motility and vigor after thawing and post thermal resistance test (TRT) were evaluated and the data were subjected to analysis of variance and means were compared by the F test at 5.0% probability. The observed values for motility and vigor after thawing and post thermal resistance test (TRT), fast and slow, according to the presence of seminal plasma and egg yolk percentage were: 5% egg yolk with plasma (25.0% and 3.3; 1.60% and 0.7; 12.36% and 1.6, respectively); 5% egg yolk without plasma (23.61% and 3.1; 1.25% and 0.2; 9.93% and 1.3, respectively); 10% egg yolk with plasma (30.8% and 3.3; 4.4% and 1.9; 19.5% and 2.7, respectively); and 10% egg yolk without plasma (13.4% and 2.5; 4.1% and 0.5; 17.0% and 1.0, respectively). There were significant differences between the analyzed data in relation to semen with or without plasma at different percentages of egg yolk, and the group that presented the best results was 10% egg yolk citrate in extender with plasma. The presence of seminal plasma and higher concentration of egg yolk in extender provide a higher viability of cryopreserved goat semen.
Documentos Relacionados
- Concentration of soybean lecithin affects short-term storage success of goat semen related with seminal plasma removal
- Clonagem, expressão e purificação de proteínas do plasma seminal bovino relacionadas à alta congelabilidade do sêmen
- Eletroforese bidimensional em gel de poliocrilamida do plasma seminal equino e a sua relação com a congelabilidade do sêmen
- Influence of seminal plasma proteins on frozen ram semen quality
- The effect of the addition of seminal plasma and antioxidants to frozen thawed ram semen.