"Efeito da colchicina na fibroses hepatica induzida quimicamente em coelhos"

AUTOR(ES)
DATA DE PUBLICAÇÃO

1999

RESUMO

The fibroses development is a fundamental point in the formation of the hepatic cirrhosis, and its comprehension is essential for the understandíng of ~the physiopatology of the cirrhosis and the consequent portal hypertension. The development of an adapted experimental model of hepatic fibroses is useful for the study of the illnesses that may develop the hepatic cirrhosis. The colchicine has been used in patients wíth hepaticcirrhosis, but its effects are not totally known. The aim of this study was to evaluate the colchicine effects in the hepatic cirrhosis chemically deveioped and to evaiuate if the Proctr Chatamra model is suitable to apply in rabbits. Thirty seven male New Zealand rabbtís were used (2.73 :t 0.05 Kg), Group A (n= 10), Group B (n= 10), Group C (n=10) and 7 controls animais. Group A was gíven only carbon tetrachroride (CCI4) and phenobarbitone (FB), Group B received the same treatment as Group A with the addition of colchicine (100 Ilg/daily/five days a week 16 weeks) and Group C received the as Group B ,but had the colchicine treatment for a longer period of time. They were given phenrr?A dissolved in drinking water (approximately 50 mg/day/animal) and a week j,,-. treatment (10% solution in com oil; 20 mg/animallw,eek) concomítant with PB.The CCI4 dose was adjusted weekly in órder to obtain serun AST and AL T levels between 400 U/I and 800 U/I. The indocianine green (lCG) pharmacokinetics were determined after injecting the ICG (8mg/Kg, i.v.) and collecting plasma samples at suitable time points for the ICG quantifcation. At the end of the experiment, animais were sacrificed, and 3 fragments of each liver sent to histology examination. A higher proportion of animais which did not receive colchicine developed cirrhosis compared to those which received the drug (8/10 vs. 0/10, p.0.01), the líver flbrosis was also reduced (p.0.01). Rabbits that had been through the 16 week treatment had at the end of experiment, ICG, y-GT, bilirrubin and weight gain similar to controls. The serum enzymes and the elimination rate of ICG (before and after 16 weeks of treatment) in the CCI4 group were: AL T: 62.6 :t 170.87 UII; AST: 39,40 :t 0.3661 mllmin/kg, respectively. The low mortality and the quantity of the animais wich developed liver cirrhosis conduce to conclude with that model is suitable. The body wight is not suitable to regulate the CCI4 doses. Only the aminotransferases measurement is necessary for control of the liver development. The colchicine reduces the development of liver fibrosis and cirrhosis in a . experimental model of chronic toxic liver damage, supporting its potential therapy of liver díseases

ASSUNTO(S)

cirrose hepatica figado fibroblasto matriz extracelular

ACESSO AO ARTIGO

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