Efeito combinado de fenofibrato e creme enriquecido com insulina na cicatrização de lesões de animais diabéticos / Combined effect of fenofibrate and enriched insulin cream on wound healing in diabetic animals

AUTOR(ES)
FONTE

IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia

DATA DE PUBLICAÇÃO

07/07/2011

RESUMO

Amputations and foot ulcerations in patients with diabetes impairs their quality of life and is also a problematic public health issue. Insulin has been topically used to treat diabetic wounds and has shown acceleration of the healing process. A previous study of fenofibrate was associated with fewer lower-limb amputations in patients with type 2 diabetes, probably through non-lipid mechanism. The aim of this study was to investigate the association and the effect of systemic fenofibrate in relation to topical insulin on wound healing in streptozotocin-diabetic rats. Diabetes was induced in rats using streptozotocin. After 4 days, diabetic animals were wounded and the wounds were treated with insulin or fenofibrate or insulin plus fenofibrate or a placebo as the control. At days 3 and 9 animals were sacrificed and wounds were excised. Therefore, the results of this study indicate that systemic treatment with oral fenofibrate in therapeutic dose does not accelerate the rate of wound healing in diabetic rats, however there is a greater activation of IR, ERK-1 in the inflammatory phase, and IR and GSK-3 in the proliferative phase, besides the improvement of the expression of TGF-ß and TNF- alfa . The combination of fenofibrate with insulin enriched cream showed an increased rate of wound healing by promoting the activation of the PI3-K, as well as increased expression of cytokines (TNF- alfa and SDF1- alfa ) and growth factors (VEGF and TGF-ß) in the inflammatory phase, and enhanced TGF-ß and TNF- alfa during the proliferative phase. We can conclude that the use of the insulin enriched cream was more effective in accelerating the healing when used alone, demonstrated by an increased inflammatory infiltrate, the maturation of collagen fibers in the dermis and angiogenesis, as well as activation of insulin signaling and the enhanced expression of SDF-1?, VEGF, TGF-ß and TNF- alfa in both the proliferative and inflammatory phases

ASSUNTO(S)

diabetes mellitus cicatrização insulina fenofibrato diabetes mellitus wound healing insulin fenofibrate

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