Dosagem de imunoglobulinas IgG e IgE totais e específicas, proteínas séricas, HDL colesterol e imunocomplexos na paracoccidioidomicose aguda e crônica

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

Paracoccidioidomycosis (PCM), caused by the dimorphic fungus Paracoccidioides brasiliensis, presents two different clinical forms: acute form (AF) and chronic form (CF). The AF occurs particularly in young individuals and is more severe and rare while the CF involves, mainly, male adults and a more benign form, in general. The more severe cases are related to the depression of cellular immune response, polyclonal activation of B cells and to a Th2 cellular immunity deviation. Several soluble components are liberated by the fungus, as gp-43, as well as the presence of circulation immune complexes (IC) has benn associated to the deviation of immune response and greater severity in PCM. The aim of this work was to determine differentiating molecular markers between the two forms of the disease. Considering the difficulties in stablishing rapid and simple serological routines with padronized antigens for ambulatory laboratory diagnosis of PCM and treatment follow-up, it was innitialy analyzed possible correlations of alterations observed in fractions of serum proteins electrophoresis and the levels of total IgG and IgE and HDL cholesterol. Thirty serum from CF CPM patients, 12 from AF PCM and 44 from healthy donors (NHS) were analyzed by electrophoresis and enzyme-linked-immunosorbent assay (ELISA). Subsequently, it was investigated the seric levels of total and specific IgG and IgE, IgG-IgE IC and IgG-gp43 IC through ELISA in sera of CF PCM (22), AF PCM (12) and NHS (29). Results demonstrated alterations in protein fractions and that there are positive correlations between beta and gamma-globulins or alpha-1 globulins, gamma and alpha-1 globulins and between gamma-globulins and total IgE levels. Through this work we have concluded that there are differences between the two forms of PCM in regard of fractions alpha-1, alpha-2 and gamma-globulins and that there is difference in albumin results interpretation when analyzed in percentages or absolute levels and that HDL cholesterol do not contribute to the elevations observed in alpha-1 globulins fraction in PCM. The results also confirm that elevated seric levels of total and specific IgG and IgE and IgG-gp43 IC are associated with the more severe form of the disease and this work evidenced for the first time the presence of IgG-IgE IC differentiating between AF and CF PCM.

ASSUNTO(S)

paracoccidioides brasiliensis imunoglobulina g imunoglobulina e microbiologia paracoccidioides brasiliensis immunoglobulin g immunoglobulin e

ACESSO AO ARTIGO

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