DNA gyrase action involves the introduction of transient double-strand breaks into DNA.

AUTOR(ES)

Mizuuchi, K

RESUMO

DNA gyrase from Escherichia coli, in the presence of ATP, can both separate catenated DNA circles and unknot knotted DNA. Both these reactions require passage of a DNA segment through a transient double-strand break in DNA. Evidence that transient double-strand breaks are also involved in the supercoiling and relaxing activities of DNA gyrase is derived from experiments showing that the linking number of circular DNA is changed in steps of two. A mechanism is proposed for the action of the enzyme.

Documentos Relacionados

• Meiotic double-strand breaks in yeast artificial chromosomes containing human DNA.
• The ubiquitin landscape at DNA double-strand breaks
• Introduction of double-strand breaks into the genome of mouse cells by expression of a rare-cutting endonuclease.
• In vitro repair of double-strand breaks accompanied by recombination in bacteriophage T7 DNA.
• Repair of Double-Strand Breaks in Bacteriophage T4 by a Mechanism That Involves Extensive DNA Replication