DNA extraction of human teeth submitted to high temperatures: evaluation of three methods . / "Avaliação de três métodos de extração de DNA de dentes humanos submetidos ao calor"

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

Biological remains of carbonized human bodies are usually not possible to use in forensic analysis. In these cases, teeth are usually selected since enamel, dentin and cement provides protection to the genetic material. The present study evaluates DNA extracted using three methods (organic, isopropilic alcohol and silica) of teeth submitted to different temperatures (200°C, 400°C, 500°C and 600°C). The method of evaluation was amplification by PCR. Two third molars of eight different individuals were used; one was kept in natura (gold standart) and the other submitted to burning. For amplification primers for genomic (STR-F13A01) and mitochondrial (MPSs) DNA were used. For genomic DNA, polyacrylamide gel analysis showed that 50% of the samples extracted with organic method were amplified, 38% with isopropilic alcohol, and 0% with the silica method. For mitochondrial DNA, amplicons sequencing showed that 100% of the samples extracted with isopropilic alcohol method were confirmed in all temperatures; 100% with the organic method in 200°C, 50% in 400°C, and 0% in 500°C and 600°C. For silica method, 50% in 400°C and 500°C and 0% in 200°C and 600°C. Our results show that the isopropilic alcohol method is the best method to extract DNA from burned teeth of all used temperatures.

ASSUNTO(S)

identificação humana dna dna forensic dentistry dna mitocondrial odontologia legal mitochondrial human identification

ACESSO AO ARTIGO

Documentos Relacionados