DNA extraction by zinc.

AUTOR(ES)

Kejnovský, E

RESUMO

A fast, very simple and efficient method of DNA extraction is described which takes advantage of DNA sedimentation induced by millimolar concentrations of ZnCl2. The zinc-induced sedimentation is furthermore strongly promoted by submillimolar phosphate anion concentrations. Within <30 min, the method recovers >90% of DNA irrespective of whether a plasmid DNA or short oligonucleotides are the extracted material. The method works with plasmid DNA and oligonucleotide concentrations as low as 100 ng/ml and 10 microg/ml, respectively, without using any expensive facilities or toxic chemicals.

Documentos Relacionados

• Zinc.
• Deletion of stably integrated DNA is suppressed by cadmium and zinc.
• Hepatocellular copper toxicity and its attenuation by zinc.
• Inhibition of DNA replication and repair by cadmium in mammalian cells. Protective interaction of zinc.
• Differential display of intestinal mRNAs regulated by dietary zinc.