Distinction of species and strains of mycoplasmas (mollicutes) by genomic DNA fingerprints with an rRNA gene probe.
AUTOR(ES)
Yogev, D
RESUMO
Genomic fingerprints of Acholeplasma laidlawii, Mycoplasma hominis, and Mycoplasma pneumoniae strains were obtained by Southern blot hybridization of the digested mycoplasmal DNAs with an rRNA gene probe. The hybridization patterns revealed genotypic heterogeneity among A. laidlawii and M. hominis strains and a remarkable degree of homogeneity among M. pneumoniae strains isolated from pneumonia patients during a 10-year period. Genomic fingerprints with the rRNA gene probe can thus serve as indicators of intraspecies genetic homogeneity or heterogeneity and can provide a new, sensitive tool for strain identification with a potential for application in epidemiology.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=266561Documentos Relacionados
- Mycoplasmas (Mollicutes) have a low number of rRNA genes.
- Genetic diversity within isolates of mutans streptococci recognized by an rRNA gene probe.
- Northern blot normalization with a 28S rRNA oligonucleotide probe.
- Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification.
- Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification.