Dissection of immediate-early gene promoters from herpes simplex virus: sequences that respond to the virus transcriptional activators.
AUTOR(ES)
Gelman, I H
RESUMO
The immediate-early promoters of herpes simplex virus give rise to the first series of transcripts after infection. These promoters are composed of compound sequence elements that govern basal level and regulated transcription. The response of three core (truncated) promoters from the herpes simplex virus type 1 IE-4, IE-0, and IE-27 genes to a battery of virus-encoded trans-acting proteins was examined in a short-term transient expression assay system. The results of this study reveal (i) a role for a sequence, 5'---GGGGG---3', flanked by 3 to 5 base pairs of symmetry (the G box), which is present in the upstream region of all immediate-early gene promoters, (ii) a requirement for the consensus sequence protected by ICP4 for autoregulation by this immediate-early gene product, and (iii) an alternative, sequence-independent mechanism for the augmentation of alpha gene expression by the virion-associated transcriptional activator Vmw65, now designated as TIF.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=255894Documentos Relacionados
- Herpes simplex virus immediate-early promoters are responsive to virus and cell trans-acting factors.
- Reiterated sequences within the intron of an immediate-early gene of herpes simplex virus type 1.
- Multimerization of ICP0, a herpes simplex virus immediate-early protein.
- Varicella-zoster virus open reading frame 10 protein, the herpes simplex virus VP16 homolog, transactivates herpesvirus immediate-early gene promoters.
- Binding of the herpes simplex virus immediate-early gene product ICP4 to its own transcription start site.