Dissecting virulence: Systematic and functional analyses of a pathogenicity island
AUTOR(ES)
Deng, Wanyin
FONTE
National Academy of Sciences
RESUMO
Bacterial pathogenicity islands (PAI) often encode both effector molecules responsible for disease and secretion systems that deliver these effectors to host cells. Human enterohemorrhagic Escherichia coli (EHEC), enteropathogenic E. coli, and the mouse pathogen Citrobacter rodentium (CR) possess the locus of enterocyte effacement (LEE) PAI. We systematically mutagenized all 41 CR LEE genes and functionally characterized these mutants in vitro and in a murine infection model. We identified 33 virulence factors, including two virulence regulators and a hierarchical switch for type III secretion. In addition, 7 potential type III effectors encoded outside the LEE were identified by using a proteomics approach. These non-LEE effectors are encoded by three uncharacterized PAIs in EHEC O157, suggesting that these PAIs act cooperatively with the LEE in pathogenesis. Our findings provide significant insights into bacterial virulence mechanisms and disease.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=373508Documentos Relacionados
- Pathogenicity and virulence: another view.
- Rabies virulence: effect on pathogenicity and sequence characterization of rabies virus mutations affecting antigenic site III of the glycoprotein.
- cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors
- Cryptococcus neoformans melanin and virulence: mechanism of action.
- Functional Transfer of Salmonella Pathogenicity Island 2 to Salmonella bongori and Escherichia coli