Direct Method for Determining the Viability of a Freshly Generated Mixed Bacterial Aerosol
AUTOR(ES)
Green, Louis H.
RESUMO
A direct method was developed to determine the viability of a freshly generated mixed bacterial aerosol. A mixed suspension of 32P-labeled Staphylococcus aureus and 35S-labeled Proteus mirabilis was nebulized, and the aerosol was collected and separated according to particle size with an Andersen sampler. Quantitative and qualitative bacteriological and radioisotopic techniques were used to obtain ratios of bacterial to radioactive counts for each organism in samples of the nebulizer suspension and aerosol. Loss of viability was calculated from the change that occurred between the ratio of the nebulizer suspension and the ratio of the aerosol. The viability of S. aureus was unaffected by aerosolization, whereas the viability of P. mirabilis declined by 20 to 60% and was inversely proportional to particle size. The advantages of this method over present indirect methods, as well as potential applications of the method, are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=547316Documentos Relacionados
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