Development and Validation of a UPLC-ESI-MS Method for Quantitation of the Anti-Alzheimer Drug Galantamine and other Amaryllidaceae Alkaloids in Plants
AUTOR(ES)
Paiva, José R. de; Souza, Ana S. Q.; Pereira, Rita de C. A.; Ribeiro, Paulo R. V.; Zocolo, Guilherme J.; Brito, Edy S. de; Pessoa, Otília D. L.; Canuto, Kirley M.
FONTE
J. Braz. Chem. Soc.
DATA DE PUBLICAÇÃO
2020-02
RESUMO
Our study aimed at the development of a method based on ultra-performance liquid chromatography coupled to mass spectrometry (UPLC-ESI-MS) using selected ion monitoring for the simultaneous quantitation of the alkaloids galantamine, pseudolycorine, sanguinine and narciclasine in Amaryllidaceae species (Hippeastrum elegans, Habranthus cf. irwinianus, Hymenocallis littoralis and Griffinia nocturna). The alkaloids were extracted from dried and ground bulbs (100 mg) using liquid-liquid microextraction followed by solid phase extraction in cation exchange cartridges. The quantification method showed good linearity (correlation coefficient, R ≥ 0.9968) and selectivity for a run time of 10 min. All values were within the acceptable limits for recovery (87.5-96.2%), interday (coefficient of variation, CV%, 1.3-8.4%) and intraday precision (CV% 5.7-8.1%), except narciclasine (70%). The limits of detection and quantification were 5-100 and 20-350 ng mL-1, respectively. Our method demonstrated to be rapid, sensitive and reliable, therefore appropriate for being employed in the prospection of new source of galantamine and other alkaloids.
Documentos Relacionados
- Seasonal Effects on HPLC-DAD-UV and UPLC-ESI-MS Fingerprints and Analgesic Activities of Vernonia Condensata Baker Extracts
- Development of a rapid method for the determination of antibiotic residues in honey using UPLC-ESI-MS/MS
- Maracujá e maçã: de resíduos ao potencial antioxidante, antimicrobiano e anti-Alzheimer
- A sensitive bioanalytical method development and validation of cabozantinib in human plasma by LC-ESI-MS/MS
- Pesticide Residues Method Validation by UPLC-MS/MS for Accreditation Purposes