Development and validation of a RP-HPLC method to determine the xanthyletin content in biodegradable polymeric nanoparticles
AUTOR(ES)
Cazal, Cristiane de Melo, Forim, Moacir Rossi, Silva, Maria Fátima G. Fernandes da, Vieira, Paulo Cezar, Fernandes, João Batista
FONTE
Quím. Nova
DATA DE PUBLICAÇÃO
2014
RESUMO
Xanthyletin is used as an inhibitor of the symbiotic fungus (Leucoagaricus gongylophorus) of the leaf-cutting ant (Atta sexdens rubropilosa), one of the most significant agricultural plague insects. The incorporation of this compound into nanoparticles is a promising approach to effectively control leaf-cutting ants. This study presents the development and validation of a specific analytical method using high-performance liquid chromatography (HPLC) for quantification of the xanthyletin content in biodegradable polymeric nanoparticles. The analytical methodology developed was specific, linear, accurate, precise, and robust. The absolute recovery of xanthyletin in colloidal suspensions was nearly 100%. The HPLC method proved reliable for the quantification of xanthyletin content in nanoparticle formulations.
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