Determinação dos dados cineticos do pre-tratamento de bagaço de cana-de-açucar com peroxido de hidrogenio alcalino e da hidrolise enzimatica posterior / Determination of kinetics data of the pretreatment of sugarcane bagasse with alkaline hydrogen peroxide and subsequent enzymatic hydrolysis

AUTOR(ES)

Daniella dos Reis Garcia

DATA DE PUBLICAÇÃO

2009

RESUMO

The objective of this work was to obtain experimental data of the kinetics of pretreatment with alkaline hydrogen peroxide and of the kinetics of the subsequent enzymatic hydrolysis of sugarcane bagasse. The pretreatment was performed in the conditions determined as optimal for this process with concentrations of 8% (w/w) of bagasse, 11% (v/v) of hydrogen peroxide and pH of 11,5. The experimental data were obtained at temperatures of 25, 45 e 65°C, analyzing the concentrations of lignin, cellulose and hemicellulose were analyzed as functions of pretreatment time. At 25°C was observed lower delignification when compared to the 65°C, but not occurred degradation of cellulose. The enzymatic hydrolysis was performed with pretreated bagasse using celulase from T. reesei and ß- glicosidase from A. Niger at 50 ºC. In the first round of experiments, bagasse mass was varied from 1 to 5 g with fixed reactional volume of 100 mL, corresponding to concentrations of 1 to 5% of bagasse (w/w). The concentrations of celulase and ß- glucosidade were fixed in 500 FPU/L and 500 CBU/L, respectively. The enzyme loading fixed difficult the transfer of mass and the adsorption of the enzymes, therefore, less satisfactory results were observed for tests with higher concentration of bagasse. Additional experiments were performed fixing bagasse mass at 3% (w/w) and varying celulase and ß- glucosidade concentrations according to a 22 + star configuration factorial design. The results confirmed that the action of enzymes on the substrate occurs in a synergistic way. In the experiments for enzymatic hydrolysis, the concentrations of celobiose, glucose, xylose and arabinose are shown as functions of time. Key-words: Hydrogen peroxide, pretreatment, hydrolysis, sugarcane bagasse

ASSUNTO(S)

hydrolysis sugarcane bagasse hydrogen peroxide hidrolise pretreatment agua oxigenada bagaço de cana pre-tratamento

Documentos Relacionados

• Evaluation of performance with alkaline hydrogen peroxide pretreatment for enzymatic hydrolisys of sugarcane bagasse
• Otimização do pré-tratamento com peróxido de hidrogênio alcalino a alta concentração de sólidos para a hidrólise enzimática de bagaço de cana-de-açúcar
• Pre-treatment and enzymatic hydrolysis of sugar cane bagasse
• Determinação de dados cineticos da deslignificação do bagaço de cana-de-açucar e da hidrolise enzimatica no pre-tratamento com hidroxido de calcio
• ELECTRON BEAM APPLICATION AS PRETREATMENT OF SUGARCANE BAGASSE TO ENZIMATIC HYDROLYSIS OF CELLULOSE