Determinação da sensibilidade de isolados de Leishmania a antimoniato de meglumina, anfotericina B e tamoxifeno. / Determination of the sensitivity of Leishmania isolates to meglumine antimoniate, amphotericin B and tamoxifen.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2009

RESUMO

In this work we evaluated the in vitro sensitivity to drugs of some isolates from Brazilian patients with cutaneous leishmaniasis. The modified MTT microtest was effective for evaluation of in vitro sensitivity of Leishmania promastigotes and macrophages from bone marrow as a model of infection by L. (V.) braziliensis. The activity of tamoxifen and amphotericin B was similar among isolates of Leishmania evaluated. Sensitivity to Glucantime®, was more variable with isolates of L. (V.) braziliensis presenting higher sensitivity to the drug. There was no correlation between clinical response to treatment with in vitro activity. We have also evaluated the effectiveness of tamoxifen in the treatment of BALB/c mice infected with L. (V.) braziliensis and observed that 20 or 30 mg/kg/day of tamoxifen for 15 days resulted in reduction in the size of lesions and parasite load when compared with control animals. One of the isolates presented atypical flagellar morphology.

ASSUNTO(S)

cutaneous leishmaniasis anfotericina b quimioterapia sensitivity and specificities tamoxifeno amphotericin b antimônio antimony sensibilidade e espeficidade chemotherapy tamoxifen parasitology leishmaniose cutânea parasitologia

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