Detection of human herpesvirus 7 infection in young children presenting with exanthema subitum
AUTOR(ES)
Magalhães, Ivna de Melo, Martins, Rebeca Vazquez Novo, Vianna, Renata Oliveira, Moysés, Natalia, Afonso, Larissa Alves, Oliveira, Solange Artimos, Cavalcanti, Silvia Maria Baeta
FONTE
Mem. Inst. Oswaldo Cruz
DATA DE PUBLICAÇÃO
2011-05
RESUMO
In this study, we assessed the prevalence of human herpesvirus-7 (HHV-7) in 141 serum samples from children less than four years of age with exanthematic disease. All samples were negative for measles, rubella, dengue fever and parvovirus B19 infection. Testing for the presence of human herpesvirus-6 (HHV-6)-specific high avidity IgG antibodies by indirect immunofluorescence assay (IFA) revealed two main groups: one composed of 57 patients with recent primary HHV-6 infection and another group of 68 patients showing signs of past HHV-6 infection. Another 16 samples had indeterminate primary HHV-6 infection, by both IgG IFA and IgM IFA. Serum samples were subjected to a nested polymerase chain reaction to detect the presence of HHV-7 DNA. Among patients with a recent primary HHV-6 infection, HHV-7 DNA was present in 1.7% of individuals; however, 5.8% of individuals tested positive for HHV-7 DNA in the group with past primary HHV-6 infection. Among the 16 samples with indeterminate diagnosis, 25% (4/16) had HHV-7 DNA (p < 0.002). We hypothesise that HHV-7 might be the agent that causes exanthema. However, a relationship between clinical manifestations and the detection of virus DNA does not always exist. Therefore, a careful interpretation is necessary to diagnose a primary infection or a virus-associated disease. In conclusion, we detected HHV-7 DNA in young children from the state of Rio de Janeiro, Brazil.
Documentos Relacionados
- Detection by polymerase chain reaction amplification of human herpesvirus 6 DNA in peripheral blood of patients with exanthem subitum.
- Productive Infection of Primary Macrophages with Human Herpesvirus 7
- Seroepidemiology of human herpesvirus 6 infection in normal children and adults.
- Human herpesvirus 7: antigenic properties and prevalence in children and adults.
- Molecular Detection of Human Calicivirus in Young Children Hospitalized with Acute Gastroenteritis in Melbourne, Australia, during 1999