Detection of Escherichia coli enterotoxins in stools.
AUTOR(ES)
Merson, M H
RESUMO
We determined whether enterotoxigenic Escherichia coli diarrhea could be diagnosed by direct examination of stools for heat-labile (LT) and heat-stable (ST) enterotoxins. The Y-1 adrenal cell and an enzyme-linked immunosorbent assay (ELISA) detected LT in 85 and 93%, respectively, of stool specimens obtained from adults with acute diarrhea from whom an LT- and ST-producing organism had been isolated. Furthermore, the ELISA assay detected LT in 8 of 35 stool specimens from which no LT-producing E. coli had been isolated. The infant mouse assay was utilized to detect ST in these stool specimens and was found to be an insensitive method, showing positive results in only 36% of the specimens from which an ST-producing organism was isolated. Further studies are warranted to determine the diagnostic value of direct detection of LT in stools, especially by the ELISA method.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=551081Documentos Relacionados
- Monoclonal antibodies for detection of Norwalk virus antigen in stools.
- Microtiter solid-phase radioimmunoassay for detection of human calicivirus in stools.
- Comparison of Rotazyme and direct electron microscopy for detection of rotavirus in human stools.
- Self-transmissible R plasmids encoding CS31A among human Escherichia coli strains isolated from diarrheal stools.
- Enrichment for Plesiomonas shigelloides from stools.