Detection of caprine arthritis-encephalitis virus by polymerase chain reaction.
AUTOR(ES)
Reddy, P G
RESUMO
Detection of caprine arthritis-encephalitis virus (CAEV) infection in goats is currently limited to serologic testing or cell culture. We developed a polymerase chain reaction (PCR) assay to detect CAEV sequences in peripheral blood mononuclear cells (PBMC), synovial fluid cells (SFC), and milk cells (MC) obtained from infected goats. Results were positive for 18 of 20 PBMC, 8 of 8 MC, and 5 of 5 SFC samples from seropositive goats, whereas 3 of 33 PBMC samples and none of 8 MC or 5 SFC samples from seronegative goats were positive. Two of the PCR-positive and seronegative goats seroconverted upon follow-up testing 2 months later. This PCR assay provides a useful method for detecting CAEV infection in goats.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=266206Documentos Relacionados
- Variability and Immunogenicity of Caprine Arthritis-Encephalitis Virus Surface Glycoprotein
- Activation of caprine arthritis-encephalitis virus long terminal repeat by gamma interferon.
- Immune responses of goats persistently infected with caprine arthritis-encephalitis virus.
- Detection of antibodies to caprine arthritis-encephalitis virus by protein G enzyme-linked immunosorbent assay and immunoblotting.
- Susceptibility of blood-derived monocytes and macrophages to caprine arthritis-encephalitis virus.