Detection of antibodies to pneumococcal capsular polysaccharides by enzyme-linked immunosorbent assay.
AUTOR(ES)
Pedersen, F K
RESUMO
An enzyme-linked immunosorbent assay for the detection of immunoglobulin G, M, and A antibodies against each of the 14 polysaccharide antigens contained in a contemporary pneumococcal vaccine is described. A mean total antibody fold increase above 2 from prevaccination to 4 weeks postvaccination serum samples was found for all antigens in 12 healthy adults and 6 children. Fifty-five percent of all single fold increases determined were above 2 in the adults; the value was 64% in children. Type-specific polysaccharide antibody of the immunoglobulin G class was predominant in 4 weeks postvaccination serum samples. Further studies with assays such as the one described may lead to a better understanding of the immune response to pneumococcal polysaccharides in both normal subjects and patients at increased risk of pneumococcal infection.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=272102Documentos Relacionados
- Immunoglobulin G and M antibodies to pneumococcal polysaccharides detected by enzyme-linked immunosorbent assay.
- Detection of antibodies to alphaviruses by enzyme-linked immunosorbent assay.
- Enzyme-linked immunosorbent assay for detection of antibodies against Streptococcus pneumoniae capsular polysaccharides.
- Enzyme-Linked Immunosorbent Assay for Quantitation of Human Antibodies to Pneumococcal Polysaccharides
- Measurement of immunoglobulin G and M antibodies to type 3 pneumococcal capsular polysaccharide by enzyme-linked immunosorbent assay.