Detecting Gene Copy Number Fluctuations in Tumor Cells by Microarray Analysis of Genomic Representations
AUTOR(ES)
Lucito, Robert
FONTE
Cold Spring Harbor Laboratory Press
RESUMO
In this work, we explore the use of representations in conjunction with DNA microarray technology to measure gene copy number changes in cancer. We demonstrate that arrays of DNA probes derived from low-complexity representations can be used to detect amplifications, deletions, and polymorphic differences when hybridized to representations of genomic DNA. The method is both reproducible and verifiable, and is applicable even to microscopic amounts of primary tumors. We also present a mathematical model for array performance that is useful for designing and understanding DNA microarray hybridization protocols. The future applications and challenges of this approach are discussed.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=310939Documentos Relacionados
- A Whole-Genome Mouse BAC Microarray With 1-Mb Resolution for Analysis of DNA Copy Number Changes by Array Comparative Genomic Hybridization
- MSX2 copy number increase and craniosynostosis: copy number variation detected by array comparative genomic hybridization
- Quantifying single gene copy number by measuring fluorescent probe lengths on combed genomic DNA
- Representational Oligonucleotide Microarray Analysis: A High-Resolution Method to Detect Genome Copy Number Variation
- Microarray analysis of tumor necrosis factor α induced gene expression in U373 human glioblastoma cells