Detecção e efeito de drogas no crescimento e desenvolvimento de formas imaturas e adultas de Chrysomya Albiceps (Wiedemann) e Chrysomia Putoria (Wiedemann) (Diptera: Calliphoridae), duas moscas varejeiras de interesse forense




The toxicological analysis of biological samples such as imature stages and adults of insects can contribute to criminal investigations to improve the estimate the post mortem interval and to determine the absence or presence of a drug in the corpse. Two blow fly species: Chrysomya albiceps (Wiedemann) and Chrysomya putoria (Wiedemann) (Diptera: Calliphoridae) were selected to the studies. Colonies of these two flies were stabilished in the laboratory. These species were chosen because they are commonly found visiting and breeding in decomposing corpses and carcasses, beeing, therefore, of forensic importance (MOURA et alli, 1997; CARVALHO &LINHARES, 2001). Diazepam, cocaine, anphepramone and marijuana were the drugs chosen for the experiments because of their heavy consuption among drug users in Brazil. Rabbits were used as test animals and were submited to twice the lethal dosages of different drugs to study the effects of these drugs on the development of the flies. Heart, liver, blood, and urine samples were taken for chromatographic analyse using a GC-MS equipment. A liver sample was used to rear the maggots and from hours 0 to 54 of exposition, larvae feeding on tissues were weighted and analised. The time required for pupariation and adult emergence was also determined. Some factors such as developmental time, mortality and longevity were also determined. All drugs influenced the developmental stages of both fly species, acelerating or delaying these stages related to control the flies. It was possible to detect the drug presence in fly and rabbit samples. The data confirm the importance of these insects as tools in criminal investigations


toxicologia drogas mosca-varejeira

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