Detecção de Lawsonia intracellularis em aves comerciais através da reação em cadeia pela polimerase (PCR) / Detection of Lawsonia intracellularis in chicken by polymerase chain reaction (PCR)

AUTOR(ES)

Cleise Ribeiro Gomes

DATA DE PUBLICAÇÃO

2007

RESUMO

Lawsonia intracellularis is an obligate intracellular bacterium responsible for proliferative enteritis in many animals, such as swine, deers, rats, hamsters, guinea pigs, rabbits, ovine, horses, foxes, dogs, ferrets, no human primats, emus and ostrichs. Currently there is no reports about the occurrence of this agent or clinical signs of its infection in chickens (Gallus gallus domesticus). The present study reports the detection of Lawsonia intracellularis at different ages of broiler breeder in four avian commercials lineages by Polymerase Chain Reaction (PCR). Of 100 faecal swabs collected from intestinal fragments, 34% were positive for Lawsonia intracellularis detection by PCR. The agent was more frequently found in chickens of 80 weeks of age presenting or not diarrhea. The intestinal fragments which faeces were positive for Lawsonia intracellularis PCR detection were submitted to histopathological exam and in the most analysed lesions were observed proliferative necrotic cronic enteritis. Although the lesions were suggestive of L. intracellularis infection by Warthin-Starry staining, the presence of the pathogen inside of enterocytes or gland cells was not observed. This results could lead to new researches about the mean of Lawsonia intacellularis in broiler industry of Brazil and the world.

ASSUNTO(S)

chicken histopatológico lawsonia intracellularis lawsonia intracellularis reação em cadeia pela polimerase histophatological aves polymerase chain reaction

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