DetecÃÃo de variantes somaclonais de bananeira âPrata AnÃâ (AAB) micropropagada / Detection of somaclonal variants of micropropagated banana plants âPrata Anaâ (AAB)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2007

RESUMO

The in vitro culture, also known as micropropagation, is a technique used in the attainment of plants with commercial value. This technique is useful in achievement for free pathogenic plants and to maintain the genetic characteristics of the mother plant. However, the micropropagation can lead to phenotypic changes in the plants, known as somaclonal variation. The somaclonal variation can reduce the production of commercial value plants, and this implies in limitations in the use of this technique. The âPrata AnÃâ banana plant presents as somaclonal variation an anomaly growth known as gigantism. The banana plant that possesses this variation produce bunches with low commercial value. However, the variation in âPrata AnÃâ is only verified after plantation. Aiming to reduce this problem the present work was carried out searching for an alternative way to differentiate normal âPrata AnÃâ banana plant from somaclonal variant ones. Morphological, anatomical and chemical markers that allowed differentiating the variants of the normal ones were used as tools. By the application of gibberellic acid (GA3) in the aerial parts of the plants in acclimatization phase it was possible distinguishing variant plants from the normal ones allowing the variant plants to grow bigger than the normal ones. Moreover, the variant plants launched leaves with the etiolated stem and foliar limb lanceolated. Data of foliar anatomy of variant and non-variant somaclonal plants of âPrata AnÃâ were measured during in vitro, acclimatization and in the field stages. The analyses were carried out to search for morphological markers that allowed to differentiate them. Comparative analyses between the normal and variant banana plant were performed to verify developmental aspects of the anatomical structures during the culture phases. Based on the results it was possible to differentiate banana plants from somaclonal variant ones based in the main vein diameter and inferior hypoderm. Analyses of mass spectrometry was also carried to distinguish plant extract from normal and variant banana plant in vitro, in the field and in the acclimatization conditions with and without GA3 application. The fingerprint of composite in the samples allowed distinguishing the somaclonal variant plants from the other ones in acclimatization phase without application of GA3.

ASSUNTO(S)

somaclonal variation espectrometria de massas micropropagations anatomia vegetal micropropagaÃÃo mass espectrometry vegetal anatomy gibberellin giberelina fisiologia vegetal variaÃÃo somaclonal

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