Desenvolvimento de um metodo de produção de silica de porosidade controlada e sua utilização na imobilização de proteinas
AUTOR(ES)
Henrique Celso Trevisan
DATA DE PUBLICAÇÃO
1993
RESUMO
This dissertation presents the results aiming the development of controlled pore siliceous supports for enzyme immobilization. At first, silicas from three sources, one prepared in laboratory and two commercially available, were submitted to hydrothermal treatment in five temperatures, the resulting products were characterized by mercury intrus10n porosimetry and their capacity of immobilizing the enzyme amyloglucosidase were evaluated. The results suggested a linear relationship of the hydro thermal treatment pressure with .the medium pore diameter and also showed that the silica prepared in laboratory was the more suitable for enzyme immobilization. Starting from the previous data, the method optimization for preparation of the silica support was initiated, being the quality of the products evaluated by measuring the water intrusion volume, mercury porosimetry and amyloglucosidase immobilization. 150-270.C, the medium pore diameter was 100-270A, relation between the temperature and the resulting was established. The last supports were characterized by mercury porosimetry and scanning electronic microscopy and the immobilization of horseradish peroxidase, amyloglucosidase, glucose oxidase, invertase, lactase and bovine serum albumin were afforded. For each protein, the optimum pore diameter for immobilization and the relation between the immobilized activity and the particle size, in this optimum diameter, was established ...Note: The complete abstract is available with the full electronic digital thesis or dissertations.
ASSUNTO(S)
enzimas imobilizadas porosidade silica
ACESSO AO ARTIGO
http://libdigi.unicamp.br/document/?code=vtls000069868Documentos Relacionados
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