DESENVOLVIMENTO DE METODOLOGIAS ELETROANALÍTICAS PARA A DETERMINAÇÃO DE ZIDOVUDINA, LAMIVUDINA E ZALCITABINA / DEVELOPMENT OF ELECTROANALYTICALS METODOLOGY FOR DETERMINATION OF ZIDOVUDINE, LAMIVUDINE AND ZALCITABINE

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

The electrochemical behaviour and the development of an analytical methodology for the zidovudine (AZT), lamivudine (3TC) and zalcitabine (DDC)determination in drugs using voltammetrics techniques were the objectives of the current study. These drugs have been extensively used in the treatment of HIV patients because its antiretroviral activity. The AZT, 3TC and DDC are reduced at a hanging mercury drop electrode (HMDE). The influence of electroanalytical parameters such as, scan rate, amplitude, nature of the support electrolyte and pH in the signal was verified to select the best conditions for quantifying these drugs in pharmaceutical forms. The obtained results had showed AZT, in solution of pH 8.0 of phosphate buffer, with only one reduction in -970 mV vs Ag/AgCl (3 mol L-1). This peak is due to reduction of the azido group present on zidovudine molecule. The linear range of standards is from 0.25 to 1.25 mg L-1. The electrodic mechanism involving the participation of 4 electrons and 2 protons in the reduction of AZT molecules. News studies were development for lamivudine and zalcitabine determination by differential pulse voltammetry in solution of pH 2.0 of Clark-Lubs buffer, with only one reduction in - 1160 and -1180 mV, respectively. Lamivudine has been the linear range of standards from 2.28 to 8.85 mg L-1 and zalcitabine from 10.0 to 23.3 mg L-1. The mechanism of these drugs involving the participation of the same number of electrons and protons. All these analytical methods were validated.

ASSUNTO(S)

zalcitabina voltammetry antiretrovirais lamivudine voltametria zalcitabine zidovudina lamivudina zidovudine antiretroviral

ACESSO AO ARTIGO

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