Cytotoxicity and bacterial membrane destabilization induced by Annona squamosa L. extracts
AUTOR(ES)
PINTO, NÍCOLAS C.C., SILVA, JUCÉLIA B., MENEGATI, LAURA M., GUEDES, MARIA CLARA M.R., MARQUES, LUCAS B., SILVA, THIAGO P. DA, MELO, ROSSANA C.N. DE, SOUZA-FAGUNDES, ELAINE M. DE, SALVADOR, MARCOS J., SCIO, ELITA, FABRI, RODRIGO L.
FONTE
An. Acad. Bras. Ciênc.
DATA DE PUBLICAÇÃO
14/08/2017
RESUMO
ABSTRACT This study aimed to further investigate the cytotoxicity against tumor cell lines and several bacterial strains of Annona squamosa and its mode of action. Methanol extracts of A. squamosa leaves (ASL) and seeds (ASS) were used. ASL showed significant antibacterial activity against S. aureus, K. pneumoniae and E. faecalis with MIC values of 78, 78 and 39 µg/mL respectively. Moreover, ASL exhibited significant biofilm disruption, rapid time dependent kinetics of bacterial killing, increased membrane permeability and significantly reduced the cell numbers and viability. Regarding the cytotoxicity against tumor cell lines, ASS was more active against Jurkat and MCF-7 cells, with CI50 1.1 and 2.1 µg/mL, respectively. ASL showed promising activity against Jurkat and HL60, with CI50 4.2 and 6.4 µg/mL, respectively. Both extracts showed lower activity against VERO cells and reduced the clonogenic survival at higher concentrations (IC90) to MCF-7 and HCT-116 lineages. The alkaloids anonaine, asimilobine, corypalmine, liriodenine nornuciferine and reticuline were identified in extracts by UPLC-ESI-MS/MS analysis. This study reinforced that A. squamosa presents a remarkable phytomedicinal potential and revealed that its antimicrobial mechanism of action is related to bacterial membrane destabilization.
Documentos Relacionados
- Physiological and anatomical alterations in Annona muricata L. and Annona squamosa L. seedlings submit by salt stress
- Anthelmintic acetogenin from Annona squamosa L. Seeds
- Perfil cromatográfico dos extratos brutos das sementes de Annona muricata L. e Annona squamosa L. através da cromatografia líquida de alta eficiência.
- Propagação por enxertia dearaticum (annona crassiflora mart.) e atemoia (annona squamosa l. x annona cherimola mill.) em diferentes porta-enxertos de annonaceae
- Phenological characterization and temperature requirements of Annona squamosa L. in the Brazilian semiarid region