Cross-linking of nucleosomal histones with monofunctional imidoesters.

AUTOR(ES)

Wyns, L

RESUMO

Cross-linking experiments with the MONOfunctional imidoester methyl-acetimidate, in the pH range 7.0 - 8.0, on rat liver nucleosomes generate a cross-linking pattern almost identical with the one observed for much longer BIfunctional reagents (e.g. dimethylsuberimidate). Combined cross-linking and trypsin digestion experiments suggest that all or at least the great majority of this cross-linking occurs on trypsin digestible segments (or "tails") of the histones. The formation of oligomers over such extremely short distances and especially the observation of an H3 homodimer suggests a very close proximity of half-nucleosomes.

Documentos Relacionados

• Photochemical cross-linking of histones to DNA nucleosomes.
• Interstrand cross-linking reaction in triplexes containing a monofunctional transplatin-adduct.
• Cross-linking reconsidered: binding and cross-linking fields and the cellular response.
• Chemical cross-linking of Chlamydia trachomatis.
• Hybridization triggered cross-linking of deoxyoligonucleotides.