Cross-inhibition between nicotinic acetylcholine receptors and P2X receptors in myenteric neurons and HEK-293 cells
AUTOR(ES)
Decker, Dima A.
FONTE
American Physiological Society
RESUMO
The enteric nervous system (ENS) controls gut function. P2X receptors and nicotinic acetylcholine receptors (nAChRs) are ligand-gated cation channels that mediate fast synaptic excitation in the ENS. Close molecular coupling in enteric neuronal membranes contributes to a mutually inhibitory interaction between these receptors; this effect is called cross-inhibition. We studied the molecular mechanisms responsible for cross-inhibition. Whole cell patch-clamp techniques were used to measure P2X- and nAChR-mediated currents in cultured enteric neurons and HEK-293 cells. In cultured myenteric neurons, ACh (3 mM) and ATP (1 mM) coapplication evoked an inward current that was only 57 ± 6% (P < 0.05) of the predicted current that would have occurred if the two populations of channels were activated independently. In HEK-293 cells coexpressing α3β4 nAChR/P2X2 receptors, coapplication of ATP and ACh caused a current that was 58 ± 7% of the predicted current (P < 0.05). To test the importance of P2X subunit COOH-terminal tail length on cross-inhibition, P2X3 and P2X4 subunits, which have shorter COOH-terminal tails, were studied. Cross-inhibition with α3β4 nAChRs and P2X3 or P2X4 subunits was similar to that occurring with P2X2 subunits. P2X receptor or α3β4 nAChR desensitization did not prevent receptor cross-inhibition. These data indicate that the α3β4-P2X receptor interaction is not restricted to P2X2 subunits. In addition, active and desensitized conformations of the P2X receptor inhibit nAChR function. These molecular interactions may modulate the function of synapses that use ATP and ACh as fast synaptic transmitters in the ENS.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2697951Documentos Relacionados
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