Cooperation between the Hemagglutinin of Avian Viruses and the Matrix Protein of Human Influenza A Viruses
AUTOR(ES)
Scholtissek, Christoph
FONTE
American Society for Microbiology
RESUMO
To analyze the compatibility of avian influenza A virus hemagglutinins (HAs) and human influenza A virus matrix (M) proteins M1 and M2, we doubly infected Madin-Darby canine kidney cells with amantadine (1-aminoadamantane hydrochloride)-resistant human viruses and amantadine-sensitive avian strains. By using antisera against the human virus HAs and amantadine, we selected reassortants containing the human virus M gene and the avian virus HA gene. In our system, high virus yields and large, well-defined plaques indicated that the avian HAs and the human M gene products could cooperate effectively; low virus yields and small, turbid plaques indicated that cooperation was poor. The M gene products are among the primary components that determine the species specificities of influenza A viruses. Therefore, our system also indicated whether the avian HA genes effectively reassorted into the genome and replaced the HA gene of the prevailing human influenza A viruses. Most of the avian HAs that we tested efficiently cooperated with the M gene products of the early human A/PR/8/34 (H1N1) virus; however, the avian HAs did not effectively cooperate with the most recently isolated human virus that we tested, A/Nanchang/933/95 (H3N2). Cooperation between the avian HAs and the M proteins of the human A/Singapore/57 (H2N2) virus was moderate. These results suggest that the currently prevailing human influenza A viruses might have lost their ability to undergo antigenic shift and therefore are unable to form new pandemic viruses that contain an avian HA, a finding that is of great interest for pandemic planning.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=135889Documentos Relacionados
- Functional Interactions between the Fusion Protein and Hemagglutinin-Neuraminidase of Human Parainfluenza Viruses
- Functional interactions between the fusion protein and hemagglutinin-neuraminidase of human parainfluenza viruses.
- Mitogenicity of influenza hemagglutinin glycoproteins and influenza viruses bearing H2-hemagglutinin.
- Proprotein-processing endoproteases PC6 and furin both activate hemagglutinin of virulent avian influenza viruses.
- Influenza virus hemagglutinin and neuraminidase glycoproteins stimulate the membrane association of the matrix protein.