Conjugation to gold nanoparticles enhances polyethylenimine's transfer of plasmid DNA into mammalian cells
AUTOR(ES)
Thomas, Mini
FONTE
National Academy of Sciences
RESUMO
Branched polyethylenimine (PEI) chains with an average molecular mass of 2 kDa (PEI2) have been covalently attached to gold nanoparticles (GNPs), and the potency of the resulting PEI2–GNPs conjugates as vectors for the delivery of plasmid DNA into monkey kidney (COS-7) cells in the presence of serum in vitro has been systematically investigated. The transfection efficiencies vary as a function of the PEI/gold molar ratio in the conjugates, with the best one (PEI2–GNPII) being 12 times more potent than the unmodified polycation. This potency can be further doubled by adding amphiphilic N-dodecyl–PEI2 during complex formation with DNA. The resulting ternary complexes are at least 1 order of magnitude more efficient than the 25-kDa PEI, one of the premier polycationic gene-delivery vectors. Importantly, although unmodified PEI2 transfects just 4% of the cells, PEI2–GNPII transfects 25%, and the PEI2–GNPII/dodecyl–PEI2 ternary complex transfects 50% of the cells. The intracellular trafficking of the DNA complexes of these vectors, monitored by transmission electron microscopy, has detected the complexes in the nucleus <1 h after transfection.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=170885Documentos Relacionados
- Enhancing polyethylenimine's delivery of plasmid DNA into mammalian cells
- The pR UV+ plasmid, transfected into mammalian cells, enhances their UV survival.
- Minichromosome assembly of non-integrated plasmid DNA transfected into mammalian cells.
- DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
- Plasmid Transfer into the Homoacetogen Acetobacterium woodii by Electroporation and Conjugation