Conjugal Transfer of Plasmid-Encoded Determinants for Bacteriocin Production and Immunity in Lactobacillus acidophilus 88 †

AUTOR(ES)
RESUMO

Lactobacillus acidophilus 88 produced a bacteriocin, designated lactacin F, that demonstrated inhibitory activity toward L. acidophilus 6032, L. lactis 970, L. helveticus 87, L. bulgaricus 1489, L. leichmanii 4797, L. fermentum 1750, and Streptococcus faecalis 19433. Production of lactacin F was pH dependent and could be maximized in MRS broth cultures maintained at pH 7.0. Lactacin F was heat stable and sensitive to ficin, proteinase K, trypsin, and Bacillus subtilis protease. L. acidophilus 88 harbored plasmids of 4 and 27 megadaltons. Variants of L. acidophilus 88 which were deficient in lactacin F production (Laf+) and lactacin F immunity (Laft) retained the two resident plasmids. A Laf− Lafs derivative, L. acidophilus 89, was used as a recipient in agar surface mating experiments with L. acidophilus 88 (Laf+ Laft). Two types of Laf+ Laft transconjugants were recovered. One type (T-E) had acquired two plasmids of 68 (pPM68) and 52 (pPM52) megadaltons that were not detected in either the conjugal donor or the other type of Laf+ Laft transconjugants (T-89). Laf+ and Laft were unstable in the plasmid-bearing transconjugant. Plasmid analysis of Laf− Lafs variants revealed that pPM52 and pPM68 were cured with loss of Laf+ and Laft. Bacteriocin production and immunity phenotypes were genetically stable in Laf+ Laft transconjugants not harboring pPM52 and pPM68, suggesting chromosomal integration of the transferred determinants. The data demonstrated intragenic conjugation in L. acidophilus and provided direct evidence for involvement of transient plasmid determinants in Laf+ and Laft.

ACESSO AO ARTIGO

Documentos Relacionados