Comunidades microbianas em canais radiculares e abscessos periapicais agudos e suscetibilidade de algumas bacterias anaerobias estritas isoladas / Microbial communities in root canals and acute periradicular abscess and antimicrobial susceptibility of some strict anaerobes

AUTOR(ES)

Francisco Montagner

DATA DE PUBLICAÇÃO

2010

RESUMO

The aim of this study was to examine the profile of microbial communities in necrotic root canals (RC) and acute periradicular abscesses (AP) in paired samples. The antimicrobial susceptibility was determined with E-test for strict anaerobes. Paired samples of RC and PA exudates were collected from 20 subjects and analyzed by terminal restriction fragment length polymorphism (t-RFLP), microbial culture, PCR, and Nested-PCR. Statistical analysis was performed. RC and PA were not different in the number of species or in the community diversity, however very few species were shared between the two communities. No single t-RF fragment was detected in all samples, and the majority was detected in only one sample. Strict anaerobes were frequently isolated in RC and PA samples, comprising the genus Prevotella spp., and the species Parvimonas micra, Gemella morbillorum e Bacteroides ureolyticus species. Porphyromonas endodontalis, Prevotella nigrescens, Filifactor alocis, T. socranskii e T. denticola were frequently detected in the RC and PA. Only one RC sample harbored E. faecalis which was detected with molecular methods. The E-test showed that at least one resistant strain was isolated from 45% of the subjects. Amoxicilin, amoxicilin + clavulanic acid and penicilin-G were the most effective antimicrobial agents, and high resistance levels were observed for erythromicin and azithromicin. PA isolates had lower MIC than RC strains, except for pennicilin-G. In conclusion, the microbial profiles of the RC and PA communities are distinct and diverged between all subjects. The heterogeneous profiles RC and PA produced microbial interactions that affected the susceptibility to the most frequently prescribed antimicrobial agents in Endodontics.

ASSUNTO(S)

endodontia reação em cadeia polimerase endodontics polymerase chain

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