Comparison of HeLa 229 and McCoy cell cultures for detection of Chlamydia trachomatis in clinical specimens.
AUTOR(ES)
Thewessen, E A
RESUMO
Consecutive clinical specimens of Chlamydia trachomatis (1,048) were inoculated in parallel on DEAE-dextran- and cycloheximide-treated HeLa 229 cells and cycloheximide-treated McCoy cells. HeLa 229 cell culture detected 113 positive specimens, and McCoy cell culture detected 103 positive specimens. This difference is not significant. However, HeLa 229 cell culture yielded significantly more inclusions than McCoy cell culture in the 95 specimens positive in both cell types (P = 0.042). For routine diagnostic purposes, a choice for one of the cell types may be determined by local preferences.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=267569Documentos Relacionados
- Detection of Chlamydia trachomatis inclusions in McCoy and HeLa-229 cells: an alternative staining technique using toluidine blue.
- Selective translocation of annexins during intracellular redistribution of Chlamydia trachomatis in HeLa and McCoy cells.
- Detection of Clostridium difficile toxin with McCoy cell monolayers and cell suspensions and comparison with HeLa cell assay.
- Comparison of various McCoy cell treatment procedures used for detection of Chlamydia trachomatis.
- Comparison of the sensitivity of primary isolation of Chlamydia. In irradiated McCoy cells or HeLa 229 cells treated with diethylaminoethyl-dextran (DEAE-D).