Comparison of four molecular approaches to identify Candida parapsilosis complex species

AUTOR(ES)

Barbedo, Leonardo Silva, Figueiredo-Carvalho, Maria Helena Galdino, Muniz, Mauro de Medeiros, Zancopé-Oliveira, Rosely Maria

FONTE

Mem. Inst. Oswaldo Cruz

DATA DE PUBLICAÇÃO

16/02/2017

RESUMO

Since the description of Candida orthopsilosis and C. metapsilosis in 2005, several methods have been proposed to identify and differentiate these species from C. parapsilosis sensu stricto. Species-specific uniplex polymerase chain reaction (PCR) was performed and compared with sequencing of the D1/D2 region of the LSU 28S rDNA gene, microsatellite typing of C. parapsilosis sensu stricto, and PCR-restriction fragment length polymorphism patterns in the ITS1-5.8S-ITS2 region of the rDNA gene. There was agreement between results of testing of 98 clinical isolates with the four PCR-based methods, with 59 isolates identified as C. parapsilosis sensu stricto, 37 as C. orthopsilosis, and two as C. metapsilosis.

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