Cloning and the nucleotide sequence of rat glutathione S-transferase P cDNA.
AUTOR(ES)
Suguoka, Y
RESUMO
A cDNA library prepared from poly(A)+ RNA of 2-acetylaminofluorene (AAF) induced rat hepatocellular carcinoma was screened by synthetic DNA probes deduced from a partial amino acid sequence of glutathione S-transferase P subunit that had been isolated from the tumor by two-dimensional gel electrophoresis. One of the four clones analyzed contained an mRNA region encoding the total amino acid sequence of this enzyme subunit and the complete 3'-noncoding region. The nucleotide sequence indicates that this enzyme subunit has 209 amino acids (calculated Mr=23,307) distinct from other glutathione S-transferase subunits such as Ya and Yc. Comparison of the amino acid sequences between these proteins indicates that glutathione S-transferase P subunit gene has been evolved from the ancestral gene at an earlier stage than the separation of Ya and Yc and that there are at least three domains having a considerable homology with each other in these enzymes. The very large increase of this mRNA in chemically induced hepatocellular carcinoma suggests a characteristic derepression of this gene during hepatocarcinogenesis.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=321937Documentos Relacionados
- Cloning and sequence analysis of a cDNA for a rat liver glutathione S-transferase Yb subunit.
- Cloning and sequence analysis of a cDNA plasmid for one of the rat liver glutathione S-transferase subunits.
- A cDNA sequence coding a class pi glutathione S-transferase of mouse.
- A Caenorhabditis elegans cDNA that encodes a product resembling the rat glutathione S-transferase P subunit.
- The human liver glutathione S-transferase gene superfamily: expression and chromosome mapping of an Hb subunit cDNA.
