Cloning and molecular characterization of the B subunit of Escherichia coli heat-labile enterotoxin.
AUTOR(ES)
Clements, J D
RESUMO
We have constructed a plasmid containing the gene for production of the B subunit of the heat-labile enterotoxin (LT-B) from a human isolate of Escherichia coli, strain H10407. The 0.8-kilobase gene fragment encoding synthesis of LT-B was cloned onto plasmid pBR322 after sequential digestion of the enterotoxin plasmid of strain H10407 with restriction endonucleases PstI and HindIII. LT-B was isolated by agarose affinity chromatography from cell lysates of recombinant clones expressing the B subunit. The B subunit was isolated in its oligomeric form, was structurally identical to native B subunit when examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, dissociated to monomeric B in the presence of 5 M guanidine, was immunologically identical to native B subunit in an enzyme-linked immunosorbent assay, and contained no demonstrable A subunit in any of the assays.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=264904Documentos Relacionados
- Subunit number and arrangement in Escherichia coli heat-labile enterotoxin.
- Fusion of Escherichia coli heat-stable enterotoxin and heat-labile enterotoxin B subunit.
- Gangliosides sensitize unresponsive fibroblasts to Escherichia coli heat-labile enterotoxin.
- Immunological relationships between cholera toxin and Escherichia coli heat-labile enterotoxin.
- Alterations at the carboxyl terminus change assembly and secretion properties of the B subunit of Escherichia coli heat-labile enterotoxin.
