Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae.
AUTOR(ES)
Sass, P
RESUMO
A gene, PDE2, has been cloned from the yeast Saccharomyces cerevisiae that, when present in high copy, reverses the phenotypic effects of RAS2Val19, a mutant form of the RAS2 gene that renders yeast cells sensitive to heat shock and starvation. It has previously been shown that the RAS proteins are potent activators of yeast adenylate cyclase. We report here that PDE2 encodes a high-affinity cAMP phosphodiesterase that shares sequence homology with animal cell phosphodiesterases. These results therefore imply that the effects of RAS2Val19 are mediated through its changes in cAMP concentration.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=387126Documentos Relacionados
- Isolation and characterization of a mammalian gene encoding a high-affinity cAMP phosphodiesterase.
- The mRNA encoding a high-affinity cAMP phosphodiesterase is regulated by hormones and cAMP.
- Molecular cloning, characterization, and localization of a high-affinity serotonin receptor (5-HT7) activating cAMP formation.
- Cloning and characterization of the low-affinity cyclic AMP phosphodiesterase gene of Saccharomyces cerevisiae.
- Relationship between low- and high-affinity glucose transport systems of Saccharomyces cerevisiae.