Characterization of red propolis : its botanical origin and the seasonal effect on its chemical composition and biological activity / Caracterização da propolis vermelha : sua origem botanica e o efeito sazonal sobre sua composição quimica e atividade biologica

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

Among the Brazilian propolis, new propolis not yet classified, according to Park et al., 2002 and called of red propolis, originary from Alagoas state (Northeast of Brazil), have showed interesting results in preliminary studies in our laboratories. Thus, the main objective of this work is to identify the botanical marker of red propolis, the chemical composition and biological activity of extract ethanolic of propolis (EEP) and the extract ethanolic of resin of plant (EER), verifying the effect of seasonal period on chemical composition and antimicrobial activity of EEP and EER. This objective was reached through the following methodologies: 1- observation of bee behavior in visiting vegetation next beehive; 2- comparison of chemical profile of vegetables visited by bees for collecting resin with red propolis, gotten for liquid chromatography of high efficiency in phase reverse and complementary analyses, for establishing the common characteristics between both materiaIs, aiming to identification of botanical biological marker; 3- evaluation of influence seasonal annual effect on the red propolis and its vegetation source by means of the antimicrobial activity and chemical profile, with bymonthly collections of the samples during the period of 1 year. The antimicrobial activity was evaluated by Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) and microorganism used were Streptococcus mutans UA159, Streptococcus sobrinus 6715, Staphylococcus aureus ATCC25923 e Actinomyces naeslundii ATCC 12104. The results had the same demonstrated to chemical profile between the EEP and the EER of the plant Dalbergia ecastophyllum (L.) Taub. Whose characteristic was the high relative concentration of isoflavonas 3-hidroxi-8,9 dimetoxipterocarpin and medicarpina. The chemical profiles of EEP and EER varied quantitatively according to seasonal effect The CIM varied between 15.6-125 mu g/rnL and the CBM of 31.2 - 500 mu g/rnL. One concludes that these propolis, whose botanical origin is the Dalbergia ecastophyllum, can be classified as 13° type of propolis, according to Park et al., 2002 and the EEP and EER showed high antibacterial activity which can be used for future molecule research in the control of caries dental

ASSUNTO(S)

antimicrobial isoflavones isoflavonas antimicrobianos

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