Cariogenicidade de diferentes tipos de leite : efeito da suplementaçáo com ferro

AUTOR(ES)
DATA DE PUBLICAÇÃO

2000

RESUMO

The aims of this study were to evaluate the cariogenicity of different types of milk and the cariostatic effect of iron, when supplemented to an infant formula. The research was divided into two experiments using an animal model subjected to a high cariogenic challenge. ln the both experiments female rats Wistar spf (specific pathogen free) were used. The animais were infected with Streptococcus sobrinus 6715 and dessalivated. ln experiment I, sixty Wistar rats were randomly divided into 6 groups and placed in individual suspended cages, where they received ad libitum: (1) sterilized distilled water (SDW) with 5% sucrose; (2) cow milk; (3) Nan lr_ reconstituted with SDW; (4) Nestogeno II@ reconstituted with SDW; (5) Ninho@ growth supporting reconstituted with SDW; and (6) SDW. ln experiment II, fifty were divided into 5 groups receiving ad libitum: (1) SDW with 5% sucrose; (2) human milk; (3) Ninho@ growth supporting reconstituted with SDW; (4) Ninho@ growth supporting reconstituted with SDW with 88 ppm of iron (FeSO4); and (5) SDW. ln the end of 21-experimental days, the animais were killed and evaluated according to recovered oral microbiota and caries score by using Keyes (1958) modified by Larson (1981). The analysis of the carbohydrates in the milks was performed by the use of High Efficiency Liquid Chromatography (HELC), the concentration of Fe was assessed by atomic absorption and concentration of F was analyzed using microdiffusion Taves method. The results were statistically assessed through Shapiro-Wilk and KruskalWallis. The results of experiment I showed that cow milk was the least cariogenic of ali milks, whereas Ninho@ presented the highest caries score. The percentage of S. sobrinus recovered from the oral cavity, there was no significant differences significant among the groups, except for the SDW group. ln experiment II, human milk presented the lowest cariogenic potential, followed by iron-supplemented milk. ln addition, the results of experiment II showed that iron when added to formulated-milk was capable of reducing the cariogenic potential in 50,5% (smooth surface) as well as reducing the number and the percentage of S. sobrinus recovered from the oral cavity. Thus, the use of dessalivated rat model has shown that human and cow milks did not show a significant cariogenic potential, while infant formulas showed a significant cariogenic potential. Therefore, it is concluded that iron supplemented milk can be used in the prevention of two important problems in public health, caries and iron deficiency anemia

ASSUNTO(S)

iron ferro caries dentarias caries rato como animal de laboratorio leite milk

ACESSO AO ARTIGO

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