Carbohydrate-limited Growth Kinetics of Tobacco (Nicotiana rustica L.) Callus 1

AUTOR(ES)

Hunt, Warren F.

RESUMO

Logistic curves were fitted to sigmoidal growth data obtained from tobacco (Nicotiana rustica L.) callus grown on media prepared with 0.1, 0.03, 0.01, and 0.003 m sucrose. Analysis of the growth curves indicated that final yields and specific growth rates were influenced by the initial sucrose concentration. Growth yields from the four treatments were similar (0.61 ± 0.04 gram dry tissue per gram sucrose supplied). Initial specific growth rates exhibited a Michaelis-Menten dependency on initial sucrose concentration such that the Vmax = 0.18 g g−1 day−1 and Km = 0.0037 m sucrose.

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