Caracterização molecular do Epstein-Barr vírus (EBV) em pacientes portadores de HIV, em tratamento, atendidos no sistema hospitalar do sistema penitenciário do Estado de São Paulo. / Molecular characterization of Epstein-Barr virus (EBV) in HIV patients in treatment from the hospitalar system in the penitentiary system from São Paulo State, Brazil.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2008

RESUMO

The Epstein-Barr Virus (EBV) is the only species to the genus Lymphocriptovirus that infects humans. One of the possible route for its transmission thought by contamined saliva and usually occurs in the childhood. This study analysed 165 clinical samples from HIV infected patients, treated by HARRT, attended in the Hospitalar System in the Penitentiary System from Sao Paulo State. The aim of this study was to search EBV in peripheral blood mononuclear cells by PCR, Nested-PCR and sequencing analysis. The results showed 11,51% of the analysed samples, positive for EBV. This samples, was sequenced with specifics primers from the EBNA-1 (Epstein Barr Nuclear Antigen 1) region. The samples were aligned by DNASTAR program. The aligned sequences showed the base conversion G to A in seven samples. This conversion caused no alteration in the EBNA-1 protein conformation. In the phylogenetic analysis the studied sequences with the sequences from GenBank was possible to observe two groups represented with type 1 and type 2 from EBV. 100% the samples studied was identified with the group characterized by the type 2 to EBV. So the seven samples showed the conversion, suggesting the origin of the one new subtype.

ASSUNTO(S)

virologia molecular vírus epstein-barr sequenciamento genético genetic sequency molecular virology medical virology biologia molecular molecular biology virologia médica oncogenic virus epstein-barr virus vírus oncogênico

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