Caracterização e filogenia moleculares de Acanthamoeba. / Molecular characterization and phylogeny of Acanthamoeba.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2001

RESUMO

In this work we performed the molecular phylogeny and characterization of 22 Acanthamoeba isolates, 14 Brazilian keratitis isolates and 8 from ATCC, 4 keratitis and 4 environmental isolates. In spite of the extensive genetic variability disclosed by RAPD, total genomic DNA RFLP and SSU rDNA RFLP techniques, these methods enabled us to group some isolates in well defined clusters of genetically more related organisms. Sequencing of SSU rDNA allowed inference of the phylogeny of our isolates with those present in the literature, which are distributed through 12 sequence types of this gene. Among the 17 keratitis isolates of our study, 16 presented SSU rDNA of type T4 (previously found to be strongly correlated to keratitis), and one was assigned to a new sequence type. Of the 4 isolates from ATCC whose sequences were previously undetermined, the two environmental isolates also constituted new sequence types, while the two keratitis isolates were assigned to type T4.

ASSUNTO(S)

acanthamoeba dna sequencing organism identification ribosomal rna rna ribossômico sequenciamento de dna phylogeny identificação de organismos acanthamoeba (de preferência em itálico) filogenia

ACESSO AO ARTIGO

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