Caracterização dos genótipos de rotavírus humano isolados de crianças hospitalizadas com quadros diarréicos em Salvador, Bahia. / Genotype characterization of human rotavirus isolated from children hospitalized with diarrheal episodes in Salvador -Bahia.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2004

RESUMO

Human Rotaviruses are the most important etiologic agents of acute diarrhea in infants and young children worldwide, being responsible in developing countries for a large number of pediatric hospitalizations and a high impact on infant mortality. Our aim was to characterize the genotypes of human Rotavirus found in children hospitalized with acute diarrhea in the Pediatric Hospital Prof. Hosannah de Oliveira of the UFBA, between September 1998 and November 2002 in Salvador, Bahia. Fecal samples were analyzed (n=358) by methods ELISA (EIARA) and SDS-PAGE for detection of Rotavirus. 168 (46,9%) samples were positive and 190 (53,1%) were negative. Only 17 (4,7%) samples had divergent results. Positive samples from one or two of these methods (n=168) were submitted to RT-PCR and Multiplex-Nested PCR in order to determine genotypes G and P. The characterization of 93,5% (157/168) of genotypes G and 85.7% (144/168) of genotypes P was possible. The binary combination between genotypes P and G was determined in 83,9% (141/168) of the samples, being the genotype more frequently found P1A[8]G9 in 77,4% (130/168) of the samples, followed by the genotype P1A[8]G1 in 5,9% (10/168) and P1A[8]G4 in 0,6% (1/168). The present work reports for the first time in the city of Salvador, Bahia the molecular characterization of human Rotavirus in children hospitalized with diarrheal clinical episodes, emphasizing the presence of genotype P1A[8]G9 as predominant in the region. The percentage of untypeable samples (16,1%) raises the possibility that additional genotypes may be prevalent. The continuous surveillance of Rotavirus genotypes by means of molecular characterization becomes necessary, since in this information may become especially useful in the production of future vaccines.

ASSUNTO(S)

genótipos brasil rotavirus genotypes epidemiologia rotavírus epidemiology brazil imunologia

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