Caracterização de loci com variação alélica no genoma de Leishmania braziliensis passíveis de uso em testes diagnósticos e em estudos epidemiológicos. / Characterization of loci with allelic variation in the Leishmania braziliensis genome that could be useful in diagnostics tests and epidemiological studies.

AUTOR(ES)
DATA DE PUBLICAÇÃO

2006

RESUMO

American tegumentary leishmaniasis (ATL) in Bahia, Brazil is an infectious disease caused by L. braziliensis. This species has a high degree of genetic variability, as well as a large spectrum of clinical manifestations of illness. A previous study of parasites isolated from patients from an endemic area demonstrated that the population of L. braziliensis was multiclonal and contained different clades which were associated with specific clinical outcomes. In this study, we wanted to identify the allelic variation in the DNA fragments generated by our three RAPD eletrophoretic profiles (ABI-04, A8 and ABI-18). We used six L. braziliensis isolates from different clades and typed two isolates with each RAPD profile. From the profiles, we submitted six distinct bands for sequencing(ABI-04: band B1 and B2, A8: band B1, B2, B3 and ABI-18: band B1). Of 24 samples submitted, 22 were successfully sequenced. The sequences generated from each RAPD profile separately and between the pairs of isolates were aligned and compared for: (a) nucleotide sequence identity: characterization as homologous loci; (b) the presence of polymorphisms; and (c) the nature of the detected polymorphisms. We found that the sequences for two corresponding bands of the ABI-04 RAPD protocol and for the three bands of the A8 RAPD protocol had around 90% sequence identity, that corresponded to homologous loci with some allelic variation. The detected polymorphisms were 97 SNPs, 227 indels and 08 microsatellites. We also analyzed the sequences for potential restriction digest sites to obtain polymorphic sites that could be used to develop an RFLP based typing method, and found up to 120 different sites between the nine loci. In the future, the allelic variations identified between these loci will be useful for the development of epidemiological and diagnostic tools with prognostic value on the human infections.

ASSUNTO(S)

polimorfismo genético l. braziliensis rapd allelic variation genetic polymorphism imunologia leishmaniasis leishmaniose variação alélica rapd l. braziliensis

ACESSO AO ARTIGO

Documentos Relacionados